Plasmid

Part:BBa_K165061:Design

Designed by: Aaron Glieberman   Group: iGEM08_BrownTwo   (2008-10-29)


pRS304* yeast shuttle vector, TRP1 selection


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
    Illegal NotI site found at 1904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1942
    Illegal BamHI site found at 1924
    Illegal XhoI site found at 1975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
    Illegal NgoMIV site found at 1563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3348
    Illegal SapI site found at 2265


Design Notes

No design considerations as of yet.


Source

This is a modification of the Sikorski vector was described in R. S. Sikorski and P. Hieter, Genetics, 122: 19-27 (1989).

References